The principle of chemiluminescence microplate

Chemiluminescence microplate reader principle introduced on the the Perlong medical devices network is as follows:

Microplate chemical luminescence is accompanied by a phenomenon of optical radiation in the substance during the chemical reaction process, can be divided into the direct light and indirect light. The direct light is the most simple chemiluminescent reaction, there are two key steps: i.e. excitation and radiation. As A, B two substances, chemical reaction occurs to generate C substance, the release of energy for the reaction is absorbed by the molecules of the substance C and transitions to the excited states C * is in the excitation of C * in the process back to the ground state to generate optical radiation. Where C * is the luminous body, this process due to C directly involved in the reaction, it is called direct chemiluminescence.

Indirect light emitting of the medical device of the reader, also known as the energy transfer chemiluminescent, it mainly consists of three steps: First reaction the reaction of A and B to generate excited state intermediate C * (energy donor); release when the C * decomposition energy transfer to F (energy acceptor), to make the transition to the excited state F * F are excited; Finally, when the F * transition back to the ground state, emit light.

A microplate reader chemical reactions to produce chemiluminescence phenomenon, the following conditions must be met:

The first is the reaction must provide sufficient excitation energy, by a step provided separately, because of the energy released by the first reaction step will disappear due to vibrational relaxation but can not emit light in solution;

The second is the least have a favorable reaction process, so that the energy of a chemical reaction can be a substance acceptable and to generate excited state;

The third is the excited state molecule must have a certain chemical luminescence quantum efficiency to release photons, or be able to transfer its energy to another molecule to make it into the excited state, and the release of photons.

Microplate chemiluminescence determination of substances can be divided into three categories:

The first class of material is the reactant in the chemiluminescent reaction;

The second type substance is a chemiluminescent reaction of the catalyst, a sensitizer or inhibitors;

III substances is the coupling reaction of the reactants, catalyst, the sensitizing agent.

These three substances can also be used to determine by marking by other substances, further expanding the scope of application of the chemiluminescent analysis.

Microplate chemiluminescence reaction of the light-emitting type flash (flash type) and is usually divided into two glow (glow type). Flash light-emitting time is very short, only a fraction of a second to a few seconds. Glow type, also known the luminous time from a few minutes to tens of minutes or hours to longer. Flash samples must be measured immediately, and must be accompanied by a fully automated pipetting and measurement elisa reader. Glow samples can be measured using a general-purpose instrument can also be equipped with a fully automated instrument.

Article : From Perlong medical (http://www.pl999.net/)

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