Eliasa test method (ELISA) what is the principle?

We go to hospital for a scan in the used equipment has its principle of work place, if working staff to understand the medical equipment work principle can better use instrument. Also can help workers better finish the work. Microplate reader is our life is very wide application of medical equipment, the eliasa immune adsorption test explain the basic principle of:

(a) to excess specific antibody or antigen) envelope in carrier (enzyme label plate), through the antigen antibody response to side substances (antigen or antibody) and enzyme marker (with HRP - horseradish peroxidase mark antibody or antigen) also combined with the carrier (solid phase separation), the washing to remove free enzyme marker, then add the substrate, has combined with the carrier to mark enzyme substrate color. Finally using photoelectric colorimetry, treat measuring content qualitative judgment or quantitative determination.
The above methods inspection sensitivity can achieve ng/ml level.

(2) envelope enzyme label plate: specific antigen solution to join enzyme label plate hole, 4 ℃ for the night. Then use liquid detergent washing 3 to 5 times (washing machine wash plate), this solution of specific antigen was strong adsorption in enzyme label plate micro hole surface, form solid phase antigen, residual liquid and impurity were washed away.

(3) to join measured sample liquid (patient serum) : sample in liquid if contains the remains to be measuring antibody, after incubate reaction, criterion and already envelope of antigen produce specific combination, generating coating antigen and antibody test to the complex, adsorbing in elisa reader analyzer  micro hole surface. Then again for washing, remove residual liquid and interfering substance.

Article: from perlong medical (http://www.pl999.net)
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