The testing analysis of Cardiac troponin

   Cardiac Troponin is from three different genes and the composition:
    Myocardial for troponin T (cTnT)-myosin combined with the unit
    Myocardial cTnI (cTnI)-inhibit the unit
    Muscle calcium protein C (TnC)-calcium combined with the unit

     (1) Because cTnI molecules in the blood is easily in the first end from C protease (amino acid 1 ~ 30), and again from N end (amino acid 110 ~ 210) degradation; CTnI containing two serine surface and two homocysteine residual base, the base is in the body ser protein kinase A phosphorylation, make cTnI molecular configuration change, can affect the antibodies that bind to; When 50% of the blood circulation AMI cTnI is phosphorylated form. Cysteine remnants and easy oxidation or reduction, this will also change cTnI molecular configurations. Only in the central WenDingOu preparation cTnI molecules antigen decided to cluster, and at the same time without regard to the above factors monoclonal antibodies to the heart of the release to the determination of molecules on cTnI. Kit for different antigen cTnI cluster of antibodies to identify different sites, some antibody combine part just the unstable or configuration change area, detection in the form of the blood cTnI is different also, so different from a range of kit reference, the standardization is the current cTnI presses for solution of the big problem. Select a table for stability of monoclonal antibodies reagents can get stability test results.

    (2) In the diagnosis of AMI, cTnT cTnI and the same value

    (3) Serious hemolysis will effect on the determination results, but slight hemolysis or blood fat to has no effect on the results. Some cTnI kit may affected by people of the opposite sex, a rat antibody antibodies and the influence of rheumatoid factor.

   (4) The results of analysis of plasma and serum differences, must pay attention to the requirements of the sample kit.