Microplate enzymes : application of immune determination

Enzyme which is of microplate reader were used for drug and immune mainly small molecules detection ELISA is much more widely used, and it can be used to test the project includes the following respects.

1.Pathogen and its antibodies are widely used in the diagnosis of infectious virus hepatitis virus rubella virus herpes virus rotavirus, etc.; Mycobacterium tuberculosis bacteria, such as the streptococcus helicobacter pylori and brinell coli, etc.; Parasites such as day 2 amebic plasmodium falciparum, etc.

2.Protein molecule components of immunoglobulin tumor markers (such as a child protein carcinoma antigen embryo prostate specific antigen, etc) all sorts of plasma protein isozymes (such as creatine kinase CK-MB) hormone (such as hCG FSH TSH).

The peptide hormones such as T3 T4 estrogen cortisol, etc

4. Drugs and drugs such as digoxin benzene benzodiazepines gentamycin morphine and so on

Enzyme immunoassays are highly sensitivity and specificity, almost all of the soluble antigen antibody system to detect it can all be the smallest testable value can reach ng pg level and even ria than analysis, the enzyme is the advantage of immune determination mark reagents is stable, and there is no harm radioactive, therefore, the application of the enzyme immunoassays changes with each new day, the enzyme immune new method for the determination of the new technology development but enzyme immunoassays in medical inspection in popular due to commodity kit and automatic or semi-automatic testing instrument the appearance of the enzyme were immune complex step, reagent preparation is only with difficulty to meet the requirements of reagent and standardized operation, can obtain satisfactory results

Commodity ELISA kit should include bag was good solid phase carrier enzyme combined substrates and twist and other advanced kit not only provide all reagent ingredients, and all are made into reagents application liquid, and in all kinds of reagent to add pigment, make present different color ELISA procedures, reagents used is much also, this is a convenient operation and non-ferrous reagent to reduce operating error ELISA all except to quantitatively measure instrument need in the excellent instrument (special called ELISA read the instrument) outside, washing board is also extremely useful washing machine using not only save time save work, but also to standardized operation, to small and medium-sized laboratory is practical and accessible but should pay attention to in using microplate washer before, should be first to wash the performance of the machine to check, confirm the hole cleaning effect is thoroughly, and repeat it well.

Semi-automatic and automation ELISA analyzer also is mature, and in the large and medium-sized clinical inspection laboratory automation ELISA analyzer has application has an open system (open system) and closed system (close system) two classes of the former is applicable to all 96 hole plate ELISA determination; The latter only and supporting the use of specific reagents
Immune filtration test and immune chromatography test is a simple fast single testing results of many advantages, such as no equipments, instruments, reagent stable, so much of this kind of test for emergency inspection not accurate quantitative, so mainly limited to test the normal body fluids not the substance (such as the diagnosis of infectious diseases or antigen antibody), and normal and very low content in a special case abnormal rising material (such as hCG) currently clinical inspection has carried out a project hCG of HCV and HIV, HBsAg etc, new projects are in continuous evolution

                                                                 Article : From perlong medical (www.pl999.net)