Restrict samples than many factors of reagent, in addition to the reaction, the first to be biochemical analysis and sample system restriction, can appear sometimes given sample reagent and instrument than not sample system within the scope of the samples than reagents (mainly some centrifugal biochemistry analyzer), therefore needs to be changed. Then under the restriction of the photoelectric detector biochemical analyzer, sometimes in normal test, absorbency range will more than photoelectric detector linear range.
So if we can change at will? Obviously is no good, sample reagent changes than by many factors limit.
1. The restriction by measuring method. Because change than sample reagents, can actually as concentrate or dilute the sample, so is likely to test range, so change samples than and take account of reagent detection limit. Also, sometimes more than detection range of the specimen also through change sample to concentrate or diluted reagent board of purpose.
2. Linear restriction by photoelectric detector. Because all sorts of analysis instrument of linear photoelectric detectors on the range, and when the change after samples than reagents, cause spectrophotometry is beyond the scope of linear area, cause error, so must consider analysis instrument photoelectric detector of linear range. Instead, for normal verification spectrophotometry is beyond the linear, also should consider adjusting samples than or replace reagent reagents.
3. The photoelectric detector by the restriction of the resolution. Is an important index of photoelectric detector, some optoelectronic detector resolution of 0.0001 Abs, and some only 0.001 Abs, determination coefficient for large and photoelectric detector resolution hours should intensify samples than to reduce reagent photoelectric detector error of the resolution. Also, in reducing reagent when sample than must also consider the resolution of the photoelectric detector error influence.
4. Lang "by the restriction of the beer's law. Biochemistry analyser is based on a long the basis of beer's law ", and lang "bill law established a a premise, is rare solution. Because only in a dilute solution to ignore the molecular interactions, such spectrophotometry and concentration proportion to existing relationship, so in change sample reagent than, can't unlimited change.
1. The restriction by measuring method. Because change than sample reagents, can actually as concentrate or dilute the sample, so is likely to test range, so change samples than and take account of reagent detection limit. Also, sometimes more than detection range of the specimen also through change sample to concentrate or diluted reagent board of purpose.
2. Linear restriction by photoelectric detector. Because all sorts of analysis instrument of linear photoelectric detectors on the range, and when the change after samples than reagents, cause spectrophotometry is beyond the scope of linear area, cause error, so must consider analysis instrument photoelectric detector of linear range. Instead, for normal verification spectrophotometry is beyond the linear, also should consider adjusting samples than or replace reagent reagents.
3. The photoelectric detector by the restriction of the resolution. Is an important index of photoelectric detector, some optoelectronic detector resolution of 0.0001 Abs, and some only 0.001 Abs, determination coefficient for large and photoelectric detector resolution hours should intensify samples than to reduce reagent photoelectric detector error of the resolution. Also, in reducing reagent when sample than must also consider the resolution of the photoelectric detector error influence.
4. Lang "by the restriction of the beer's law. Biochemistry analyser is based on a long the basis of beer's law ", and lang "bill law established a a premise, is rare solution. Because only in a dilute solution to ignore the molecular interactions, such spectrophotometry and concentration proportion to existing relationship, so in change sample reagent than, can't unlimited change.
All in all, only in recognizing various relations to know to don't change, can change and how to change.
Article : From Perlong medical (www.pl999.net)
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