The specific content ofmicroplate reader zero drift measurement experiment is as follows:
To take eight orifice cup, were placed in the corresponding position of the microplate eight channels are added 200 ul of distilled water and zero, dual wavelength or single wavelength (490 nm) measured once every 30 min observation eight channels the change in absorbance within 4h. With zero difference is the zero drift.
Microplate reader zero drift measurement principle:
Microplate reader zero drift is usually small, which is because the instrument, the first thing to do before reading the eight channels of the background measurements (Io). And then reads each hole measuring the value (I) of the sample plate according to Beer's law, light absorption value = 1ogl0 (Io / I) for each reading after such automatic correction, so that the reading error is greatly reduced, such readings will undoubtedly is correct; addition, some detection microplate reader application "DRL DYE" strip to the absolute absorbance calibration single wavelength measurement, will lead to a false increase of absorbance, this medical equipment take two approaches correcting First, select a suitable reference filter, dual wavelength measurement, the second is the introduction of a correction parameter, i.e., the single wavelength in the absorbance mode to read a blank hole, the test values and the predicted value The difference is the correction parameter. Therefore, zero drift is the trend of the evaluation instrument within a certain time zero absorbance, has nothing to do with the wavelength, which indirectly reflects the interior of the detection instrument system stability under the working condition and apparatus within a unit time is the mechanical properties (continuous feed plate detecting, exit), so it is rated microplate internal circuit system, an optical system (detector), and mechanical systems good indicators.
Article : From Perlong medical (http://www.pl999.net/)
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