1. The biochemical analysis method
Traditional detecting blood sugar common method of extraction from the body vein blood or punctured patient fingers, extracting blood samples, and then blood cells fully automatic analyzer for testing, belong to the test. The methods used in the detection of early blood sugar fain armour aniline method, this method to aldehydes sugar such as glucose, half of lactose, xylose even ascorbic acid are reaction, and in the acidic environment produced in green, adjacent to a specific poor aniline glucose, and acidic solution to automation determination of the pipe with destruction, so in recent years have gradually be eliminated. The glucose oxidase (GOD) method is the most widely used blood sugar test of a kind of method, this law by other compounds in the disturbances, its principle for use of oxygen and water will glucose oxidation as glucose acid, and release hydrogen peroxide. Peroxidase in color, former sex oxygen receptors will using hydrogen peroxide decomposition for water and oxygen, and make the color of oxygen receptors for amino Ann than forest and phenol to hydrogen condensation red quinone compounds, namely Trinder reaction. Red quinone compounds the relic of glucose is proportional to the content. In recent years, application has sugar kinase (HK) method, the enzyme will conversion of glucose to glucose and phosphoric acid (G6P), as have other G6P dehydrogenase (G6PD) exists, can determine the enzyme NADP ability and measure the reduction of glucose. Realize the detection system of plasma glucose determination comparability, the clinical diagnosis and treatment has a very important value, because blood sugar test method of various instruments, in addition to making every instrument interior quality control outside, still should establish the target can be trace detection system, will build the results of inspection and target detection system comparing the result, make each detection system the results between the comparable. The world health organization recommends GOD method and HK as a method for determination of glucose, the reference standards, and get the 19 th world diabetes congress and the official website of the advisory groups WHO recognized, suggest vein plasma glucose should be as measuring and reporting of blood glucose concentration standard method. Think the test result is stable, reliable, but many times determination, need to be repeated blood, determine the results relatively long time, it is not convenient to patients, this from the body draw blood biochemical analysis belongs to the test, to the patient not only bring pain, easy to cause the various body fluids the spread of infectious diseases, exsanguinate trouble, blood use, and can't see results at that time, therefore, biochemical analysis application by certain restrictions.
Traditional detecting blood sugar common method of extraction from the body vein blood or punctured patient fingers, extracting blood samples, and then blood cells fully automatic analyzer for testing, belong to the test. The methods used in the detection of early blood sugar fain armour aniline method, this method to aldehydes sugar such as glucose, half of lactose, xylose even ascorbic acid are reaction, and in the acidic environment produced in green, adjacent to a specific poor aniline glucose, and acidic solution to automation determination of the pipe with destruction, so in recent years have gradually be eliminated. The glucose oxidase (GOD) method is the most widely used blood sugar test of a kind of method, this law by other compounds in the disturbances, its principle for use of oxygen and water will glucose oxidation as glucose acid, and release hydrogen peroxide. Peroxidase in color, former sex oxygen receptors will using hydrogen peroxide decomposition for water and oxygen, and make the color of oxygen receptors for amino Ann than forest and phenol to hydrogen condensation red quinone compounds, namely Trinder reaction. Red quinone compounds the relic of glucose is proportional to the content. In recent years, application has sugar kinase (HK) method, the enzyme will conversion of glucose to glucose and phosphoric acid (G6P), as have other G6P dehydrogenase (G6PD) exists, can determine the enzyme NADP ability and measure the reduction of glucose. Realize the detection system of plasma glucose determination comparability, the clinical diagnosis and treatment has a very important value, because blood sugar test method of various instruments, in addition to making every instrument interior quality control outside, still should establish the target can be trace detection system, will build the results of inspection and target detection system comparing the result, make each detection system the results between the comparable. The world health organization recommends GOD method and HK as a method for determination of glucose, the reference standards, and get the 19 th world diabetes congress and the official website of the advisory groups WHO recognized, suggest vein plasma glucose should be as measuring and reporting of blood glucose concentration standard method. Think the test result is stable, reliable, but many times determination, need to be repeated blood, determine the results relatively long time, it is not convenient to patients, this from the body draw blood biochemical analysis belongs to the test, to the patient not only bring pain, easy to cause the various body fluids the spread of infectious diseases, exsanguinate trouble, blood use, and can't see results at that time, therefore, biochemical analysis application by certain restrictions.
2. Fast blood sugar test
In 1968 the first fast blood analyzer was published, in patients with fingertips a drop of blood can be to test your blood sugar, belong to the minimally invasive testing. The early detection principle for the glucose oxidase reaction than color. The enzyme to touch as enzyme oxidation of glucose glucose acid and hydrogen peroxide, and hydrogen peroxide oxidation and oxygen to accept agent produce color change, usable reflection or absorbed photometric were monitored. Has already been developed into the electrochemical analysis, through the reaction produces in the process that the current blood sugar. Fast blood glucose meter has the stable and reliable, simple operation, economic, health, especially for less need for blood glucose monitoring of patients many times, can reduce the pain from the venous blood in many times, and provide the scientific basis for rapid diagnosis. So are widely used for testing the commonly used tools bed blood sugar, and get good evaluation. But is the whole blood capillary blood endings, including plasma and red blood cells. And red blood cells contain less than plasma glucose, hollow when endings blood glucose levels should the whole blood plasma glucose levels than venous blood low about 12%, and with the peripheral blood often take with ZuZhiYe leak, make the endings are more likely to lower than venous blood glucose plasma glucose. The blood analyzer mainly by patients to clinical operation, the influence factors of the glucose levels increase, sometimes error is bigger, largely to the operator, of course, blood glucose meter itself quickly quality reliability and accuracy also have relations. According to the provisions of the operation, such as not calibration test code, save dipstick improper, not regularly with correction fluid correction, etc.; Not for the blood analyzer regularly cleaning maintenance, eliminate color system than by pollution; To the fingertips when disinfection, cannot use iodine, disinfection of alcohol to dry with fingertips to puncture; While testing blood sampling the drops of blood too little or squeeze the fingers too hard to ZuZhiYe dilute the blood and so on, its accuracy will be affected. In addition, vitamin C, bilirubin, there were internal or external blood interfering substances, some patients with high blood fat and blood is oily shape, can make the light reflecting error than color, such as by colorimetric fast blood analyzer will influence the results. Blood sugar too much or too little easy out error, but also by the electromagnetic interference. These various influence factors, limiting the blood of the analyzer result can not fast as diabetes diagnosis, can only be used for people with diabetes daily self monitoring, to ensure the accuracy of the results, should be timely calibration and quality control.
Article : From Perlong medical (www.pl999.net)
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