How to select microplate reader (1)

In recent years, with multi-function microplate reader  in the
domestic various universities laboratory gradually promotion to open,
and multi-functional microplate reader  brands and models also
gradually more up, gradually to spend your eyes in addition to each
brand all have their own a even more series product line, character of
each are not identical, when the choose and buy all kinds of technical
parameters technology index is mind-boggling.
        This paper try to from the user of the actual use of the
perspective, probes into how to look at the great variety of
characteristic parameters, the hope can help you find the real
appropriate oneself more reactive enzyme from inspection workstation.
I. Filter Vs grating
          Multifunctional microplate reader  classification method is
numerous, but the most simple is  using their filter way to make light
boundaries in general, could be divided into filter type and grating
type two kinds big but filter and grating and can't finish at the same
time the same testing, or want to use when the grating with grating,
the use of the filter with the filter; And some of the experiments with
one of them not, and the other a module that didn't happen so this kind
of instrument is essentially the filter and grating in together, and
not make the two produced the new breakthrough mix.
         Overall, filter technology because development already for a
long time, with two to color mirrors (in fact also is another mode
filter light reflective filter) light path system, can realize the need
to present the most conventional multi-function enzyme standard
instrument of the highest detection sensitivity is to use the filter to
make but type filter type instrument because of limited to filter
wavelengths and limit, can't satisfy the growing experiment types of
detection will need, and sometimes need to the absorption of material
excitation and emission spectrum research, so later was born grating
type instrument.

       Grating is the advent of the standard type enzymes, and the user
in the wavelength choice no longer restricted, and in the miscellaneous
light rate control performance bandwidth is beyond the filter system
these are currently filter microplate reader cannot or more difficult
to achieve.

II .  Miscellaneous light rate and wavelength accuracy
          Grating type filter system has become the general current
peremptory multi-function microplate reader  of mainstream, several
manufacturers joint efforts, has set the grating technology driven to a
record high in many of the technical parameters of grating, the key is
no doubt of the grating miscellaneous light rate and the accuracy of
the wavelength selection.
        Miscellaneous light the light source is the rate through the
grating, get light, do not need to account for the wavelengths of light
that the proportion of nominal wavelengths of light, and
characterization of the purity of the filter light because the light
interference of the complexity of the diffraction, no matter use filter
or grating, miscellaneous light is inevitable all kinds of filter the
essence of technology is light to try to remove noise as possible
light.

         Generally speaking, filter type of noise rate in light 10-4 ~
10-5 between, grating type can do 10-6 ~ 10-seven because this kind of
miscellaneous light nonspecific, but also directly into the last of the
detector, so how many mixed the light will how much random errors
introduced in fluorescence detection such as process, because detector
amplification effect exists, noise interference light rate will also be
index amplifier therefore, miscellaneous light rate is a filter
system's first performance indicators

          The two , it is grating even filters filter system the most
key technical parameters, directly affect is to get data is really to
the results of the measurement, the reliability of the experimental
results is the most basic requirements.