2012年3月11日星期日

How to select microplate reader (2)

III. the authentication > parameters
       In order to ensure reliable detection, on the basis of the
different instruments under a difference is reflected in the detection
sensitivity on top, and then people care about is the instrument to
detect more weak signal.
The light sensitivity involves road system design and material, and
it's hard to say a certain components will be a decisive role but have
a bit, in all kinds of ChanGongNeng testing project, light absorption
detection with a large photoelectric diode put fluorescence detection
with photomultiplier tubes with specially designed light detection
single-photon counting photomultiplier tubes, these three different
detector is their testing field choice of priority
       Each single detection of the optimal result is done with the
corresponding detector, of course, also some instrument for cost
consideration, use a variety of testing mode detector compatible, this
in part to finish the experiment demand, but the performance will also
have to sacrifice compromise, unable to realize all tests are to reach
optimization.
        The definition and the detection sensitivity about standard,
each manufacturer has its own description, will say that he is about
how good, so it is difficult to have an intuitive objective is so, some
reagent manufacturer stood out, the detection performance of some
instruments to provide a third party certification.
        The instrument because new type grating technology in recent
years that it has gradually arisen, limited to technology research and
development and equipment costs, the same price range and sensitivity
of index will be slightly worse than development mature filter machine
so, in the midrange multi-function enzyme standard instrument field,
got all kinds of authentication of the grating type instrument does not
see more
        It shows that the type of meter standard grating enzyme
development and to enter into a new height, except in the flexibility
has an irreplaceable role outside, still in the detection sensitivity
etc also gradually replacing the traditional filter type enzyme
standard instrument grating is scientific research fields type multi-
functional enzyme standard instrument future main trends of
development; And filter type instrument is also to individual
performance better impromotion.
IV  More than four color cup + trace detection
        In addition to regular 6 ~ 384/1536 hole enzyme panels
detection besides, some with instruments than color cup trace detection
board of compatible function whether used vertical than color cup than
color glass or horizontal various trace detection board, the purpose is
the process of light to not fixed enzyme panels detection into a
standard optical path concept though enzyme panels detection can also
through the optical path correction, etc. To realize 10 mm optical path
OD value of the conversion, but it was just a mathematical conversion
process, examination error accumulated more, actually use the effect
not beautiful introduction and trace detection than color glass plate,
light absorption of light by testing process fixed in 10 mm or the
standard length of 0.5 mm, etc, OD value conversion more simple and
accurate with such testing function of enzyme standard instrument,
equivalent to in addition to the multi-function enzyme standard testing
itself outside.

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