The principle of chemiluminescence microplate

Chemiluminescence microplate reader principle introduced on the the Perlong medical devices network is as follows:

Microplate chemical luminescence is accompanied by a phenomenon of optical radiation in the substance during the chemical reaction process, can be divided into the direct light and indirect light. The direct light is the most simple chemiluminescent reaction, there are two key steps: i.e. excitation and radiation. As A, B two substances, chemical reaction occurs to generate C substance, the release of energy for the reaction is absorbed by the molecules of the substance C and transitions to the excited states C * is in the excitation of C * in the process back to the ground state to generate optical radiation. Where C * is the luminous body, this process due to C directly involved in the reaction, it is called direct chemiluminescence.

Indirect light emitting of the medical device of the reader, also known as the energy transfer chemiluminescent, it mainly consists of three steps: First reaction the reaction of A and B to generate excited state intermediate C * (energy donor); release when the C * decomposition energy transfer to F (energy acceptor), to make the transition to the excited state F * F are excited; Finally, when the F * transition back to the ground state, emit light.

A microplate reader chemical reactions to produce chemiluminescence phenomenon, the following conditions must be met:

The first is the reaction must provide sufficient excitation energy, by a step provided separately, because of the energy released by the first reaction step will disappear due to vibrational relaxation but can not emit light in solution;

The second is the least have a favorable reaction process, so that the energy of a chemical reaction can be a substance acceptable and to generate excited state;

The third is the excited state molecule must have a certain chemical luminescence quantum efficiency to release photons, or be able to transfer its energy to another molecule to make it into the excited state, and the release of photons.

Microplate chemiluminescence determination of substances can be divided into three categories:

The first class of material is the reactant in the chemiluminescent reaction;

The second type substance is a chemiluminescent reaction of the catalyst, a sensitizer or inhibitors;

III substances is the coupling reaction of the reactants, catalyst, the sensitizing agent.

These three substances can also be used to determine by marking by other substances, further expanding the scope of application of the chemiluminescent analysis.

Microplate chemiluminescence reaction of the light-emitting type flash (flash type) and is usually divided into two glow (glow type). Flash light-emitting time is very short, only a fraction of a second to a few seconds. Glow type, also known the luminous time from a few minutes to tens of minutes or hours to longer. Flash samples must be measured immediately, and must be accompanied by a fully automated pipetting and measurement elisa reader. Glow samples can be measured using a general-purpose instrument can also be equipped with a fully automated instrument.

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Price of perspective ,medical x ray manufacturers

Generally speaking perspective machine is refers to the X-ray machine, perspective machine principle x ray is a kind of invisible light, in principle, he and infrared, ultraviolet, microwave equivalent belongs to the spectrum in dispersion, and their different lengths, collectively referred to as optical spectrum. Is through the prism scatter color get different frequencies of light. Light also can be divided into visible and invisible light. Visible light is our daily life to see all sorts of color is light, invisible light such as TV remote infrared, the sun ultraviolet ray, the microwave microwave oven, medical X-ray machine use X-ray apparatus principle.
In the perspective machine manufacturer home, she medical X-ray machine perspective machine PLX2200, the characteristics of shown below:

1, high quality combined high frequency high voltage X-ray generator, no high voltage cable design, reducing wear and tear, ray quality good, image is clear with pulse perspective.
2, the human body graphics SPC liquid crystal type touch screen control design, the operation is more intelligent, more convenient.

3, hand brake and the foot brake two kinds of control mode, flexible switch, convenient application.
4, dynamic image limit resolution 2.0 LP/mm.
5, has the perspective KV/MA automatic tracking function, make the image brightness, resolution is always in the best state.
6, high quality intensifier, high-definition low illumination CCD, non-interlaced output central control system, progressive scan display device, match

(perspective machine manufacturers - perlong medical -- Fluoroscopy units PLX2200)

PLX2200 is one of the high ratio of a new medical X-ray instrument , widely used in human esophageal, chest, gastrointestinal, abdomen, limb parts such as continuous fluoroscopic diagnosis, high market share.

- this kind of perspective machine price quotation ?
- you can directly dial web top of the free customer service hotline: 400-6656-888 consultation to understand.

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Medical equipment market of illegal medical practice, the effective purification medical equipment order

Medical equipment market existing illegal medical behavior to the health of the people, caused certain threat, such behavior in order to strengthen the regulation blow, China's many cities are constantly increase law enforcement efforts, and regulation has achieved remarkable success. As we have learned, LiQuanXian taken many measures applied, combination with "three three check SanJin three solution" theme development "catch style optimal environment promoting development" and "merit YiManYi" activities, crack down on medical equipment market illegal medical behavior, the effective purification in the county YiXie market order.

According to the report, the LiQuanXian check medical institutions 397, banning illegal medical 3 door, confiscated drugs and box, medical equipment and parts, a fine of 40000 yuan, demolition of illegal medical advertisement 11, accept masses on three charges.

Designation professional technicians to the township health center rotation ZuoZhen 40 people, to carry out medical technology lectures four times, support equipment more than 10 Taiwan, optimize medical environment, improve the quality of medical treatment service purpose. On this basis, LiQuan from optimization service principle of archives, the implementation of the cover of the new rural cooperative medical care, aged 65 years and over the elderly health physical examination, the maternal free hospital delivery project, mother health engineering, children's planned immunity, infectious disease management and so on a number of basic health care projects, through the county community health service center (station), rural hospitals, village clinic and urban and rural base layer medical institutions provide free for all residents, effectively improve the nation's health care consciousness, from start early prevention, the disease nipped in the bud. To realize the goal of everyone go to a doctor is guaranteed.

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Tips of Domestic Medical Equipment Sales

In recent years, because of rapid development ofChina's medical equipment industry, many manufacturers mushroomed up. But taking a share is not an easy task in the environment of the medical reform. At present the majority of manufacturerme are using the traditional medical equipment sales, the manufacturer of the equipment sold to hospitals integrate resources of agents, its drawback is that (to go through the department director, Equipment Division, vice president of the hospital into a single-cycle long, tender and other sectors), high costs, fierce competition, low profit margins, there are many drawbacks of this way of selling, there are many of the medical equipment market place NA. What are the common effect of a good way of selling it?

 1. Cooperation divided into. Some hospital procurement of medical equipment, but the moment can not even pay for, take the form of cooperation into the medical equipment vendors, such as 50 million of equipment, the hospital first pay $ 100,000, then shared equally, when the amount of suppliers into reached 40 million, the contract is terminated, and equipment owned by the hospital all.

This sales approach is conducive to avoid vicious competition, without bidding, the drawback is that risk, financial pressure, and suppliers to sign such a contract is very cautious, usually the contract price is the selling price of 2.5 times, and the suppliers up and down and hospitals have good relationship. Most importantly, there is no change in the life of the contract, the hospital senior staff.

Medical items packaged. After 2005, with the medical equipment sales increasing competition, some hospitals for rating or a new building completed, you need to purchase a batch of equipment. Some medical equipment dealers transformation test the water "medical items packaged together, the department needs the equipment packaged and sold to the hospital.

This way of selling on the bargaining power of hospitals, turnover, some hospitals are more willing to accept the simple, efficient mode. Dealers do "medical items packaged in Beijing, Shanghai, Jiangsu, Anhui and other places, has been formed.

 3. Equipment leasing. With the development of medical science and biological engineering technology, hospital surge in demand for high-end medical equipment, such as MRI, CT, PET, Gamma Knife and other high-tech imaging equipment and radiation therapy equipment, but these devices often require several million dollars , even thousands of yuan funds. The hospital is the moment to take so much money, how to do? Introduction of financial leasing companies.

 It is a way of selling a win-win situation. Abroad, the finance lease is already very mature, and a wide range of financial instruments, but many domestic users, especially in hospitals, is still relatively fresh things. Currently, nearly 1/3 of the global investment in finance leases completed with the banking, insurance, trust, securities tied for the top five financial pillar. It is reported that in 2003 the global leasing total of $ 461.6 billion, the United States, Japan, Germany, respectively, at $ 204 billion, $ 62.1 billion, $ 38.8 billion in the first three, the lease amounted to only $ 2.2 billion.

 Medical equipment investment. Medical device investment is different from the cooperation into, the difference is that the cooperation divided into still stay in the level of selling equipment, medical equipment investment is integration, complete, comprehensive solutions. It not only provides medical training, and product maintenance and other support services, in order to better realize the value of the product, faster, and will also provide marketing, project management, promotional marketing and other related services, so that customers do not have to worry about when the operators of the new project.
Above several medical device sales methods have their own advantages and shortcomings, according to the specific circumstances, to choose the right way of selling. Only the right, is the best.

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Four breakdown solutions introduction of microplate washer

Microplate washer will often appear fault what? To meet these failure need how to deal with?

In the use of various types of washing machine in some common attention, washing machine such as boot check lotion bottle, liquor bottle, water jet needle, suction needle and so on various parts are working properly, if there is a problem should be timely solve.

First, note liquid pipeline not clear
1, the performance of fluid jet strength is insufficient, part of the note liquid pipeline even jam.

2, causes, and treatment
(1) lotion crystallization jam note fluid tube, first off nozzle, reoccupy supporting cleaner poke out tamper.
(2) infusion conduit ageing deformation, joint closes lax, cause leakage, leakage. Should be in duct, tightening joint.

Second, suction fluid pipe not clear
1, performance liquid suction cup after the hole have residual fluid, note after fluid hole in the spill.
2, causes, and treatment
(1) suction fluid tube was fibrin clot, wastepaper and solid jams, processing method to unload nozzle, reoccupy supporting cleaner poke out tamper. Work should be completely and blood coagulation precipitation serum, or with plasma, avoid fiber protein precipitation; Clappers from wastepaper adhesion, anyhow eliminate cutting may cause suction fluid pipe blockage factor.
(2) infusion conduit ageing deformation, joint closes lax, cause leakage, leakage. Should be in duct, tightening joint.

Third, overflow liquid
1, performance imbibing clean but note after fluid lotion overflow cup hole.
2, reasons and processing programming note liquid quantity in cup hole capacity. Modify the program, adjust the filler amount, with the cup of hole capacity phase matching.

Fourth, irrigating needle stuck enzyme label plate (fit eliasa use medical instrument)
1, performance wash the board irrigating needle stuck enzyme label plate, enzyme label plate carrying frame can not continue movement.
2, causes, and treatment
(1) specimen quantity for a little while different project enzyme mark strip in a enzyme label plate rack, each project enzyme mark batten placed rugged, and irrigating needle rising height is certain, high hole stuck irrigating needle. At this time should suspend wash plate, the plate will force higher pore pressure flat.
(2) support lotion needle lift spring aging, stretch inadequacy, flush plate rising height is too low. Should be replaced spring.
As long as the correct use and maintenance of washing machine, appear afore-mentioned small fault promptly eliminate, washing machine will make our enzyme from inspection workstation both relaxed and free from contamination.

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How to choose surgical CPAP system , must be constant pressure type of surgical ventilator machine

Surgical CPAP system , neonatal respiratory machine when the choice must be constant pressure type breathing machine this kind of medical apparatus and instruments, because want to protect guard neonatal fragile alveolar, try not to hurt.

Neonatal pulmonary function development incomplete, the bigger breathing work, will affect neonatal rescue. Therefore, the newborn breathing machine, from the hardware circuit and the design way, as far as possible for neonatal physiological features: die cavity quantity is small, to nearly the patient flow sensor, warming humidification to have assurance. In order to reduce neonatal respiratory work, increase of vent Ann all sex. In the software design, want to have more alarm Settings to arrive when mechanical ventilation system safety.

Therefore, in order to take care of the newborn unique physiological characteristics, in new pediatric NICU choose breathing unit, should choose in gas path and circuit, software and hardware are accord with neonatal characteristics ofpediatric breathing machine. Hospital department of segmentation, is the sign of the development of medical equipment , the new special breathing machine choice, and the increase of the level of the hospital's medical an important sign.

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Pay more Attention to Four-inspection Work of Blood Cell Analyzer

Operators should pay more attention to four-inspection work of blood analyzer. And the specific description is as follows:

The blood cell analyzer known as the blood cell analyzer, blood analyzer is commonly used in the medical field, medical devices, and the use of the blood cell analyzer greatly improve the quality and efficiency of clinical blood tests. However, this type of high-tech medical devices in the identification of blood cell morphology and structure is not perfect, currently available only as a whole blood cell analysis sifted means. Must also be met in doubt, review of blood under the microscope piece after confirmation, amendment or supplement to issue a report. Otherwise, it is easy to cause misdiagnosis. Standard of review, the existing textbooks and operational procedures of the medical examination are not explicitly discussed. To this end, we practice and experience, combined with the literature at home and abroad, its standards, content, and methods for preliminary study.

Review the contents of the

Many people think that the review is for leukocyte classification, it is not true. The review should include observation of the morphology of the red blood cells, white blood cells and platelets; estimated platelet or white blood cell count (confirmed with the instrument given data are consistent); observe whether platelet aggregation, or red blood cell aggregation as well as any special form of abnormal cells and parasites.

Review the standard

Want to review the case, so far there is no unified standard, it was suggested that as long as the blood cell analyzer gives warning signal (FLAG), should review, this practice is a bit too wide to grasp its scope. For example, a traumatic acute hemorrhagic patients, blood analyzer gives red blood cells and hemoglobin low signal, it does not necessarily have to review the blood film. Another example is often in the course of treatment for patients with hematology tests often do not have every time review. Recommended that each laboratory should be self-imposed review of the parameters, the histogram or scatter plot; appear blood cell analyzer run prompt signal or explanatory prompts signal, etc., are to review one of the conditions, but in the exposition of the blood film review standards be considered altogether blood cell analyzer status and the patient's condition.

Review

First observed with low magnification or high magnification, the blood tablets and cell distribution, whether platelet or red blood cell aggregation (clusters, piles), tail whether the large piles of abnormal cells; continue oil lens in the smear The medium thickness Department browser blood slides, careful observation of the morphology of the red blood cells, white blood cells and platelets, and to estimate the quantity. If the report consistent with the observed results with the instrument, and not require any additional testing, the results can be measured by the instrument issued the report.

Such as anemia or other blood disease, the red blood cell count and related indicators (such as MCV, MCH, MCHC), red blood cell volume distribution width (RDW), histogram or scatter plot abnormal Emphasis was placed on the size of red blood cells, morphology of inclusion, coloring, size, consistency, and presence of nucleated red blood cells, etc.. If abnormal, should be described and reported. Blood in vitro storage too long or smear when other technical reasons, and sometimes the local area of ​​the blood spots may appear fake target-shaped red blood cells, lip red blood cells and leave spherocytosis, testing often lack the experience will be their when really shaped red blood cells. The most simple method of identifying Browse other regions of the smear, and if it is really shaped red blood cells, the entire film (instead of individual regions) can be seen in the same exception.

Review

Personnel engaged in the review of blood films must be professionally trained basic and clinical knowledge of blood cell morphology, and very familiar, especially familiar with the various histograms, scatter plots of normal and abnormal pattern of blood cell analyzer, and abnormal pattern significance interpretation and assessment of personnel.

Review by first carefully read the given parameters of hematology analyzer, histograms, scatter plots and prompt signal. Hematologic abnormalities that may exist or technical impact factors have an initial impression. Combined with the patient's clinical situation (including the initial diagnosis, etc.), to determine the review focus.

Thoroughly mixed blood as soon as possible to push into the blood slides and stained. Skin puncture blood the EDTA anticoagulant smear Unlike direct smears are some differences in cell morphology; addition, most of the anti-clotting in vitro saved some time will have an impact on cell morphology.

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