We Perlong Medical was founded in 1993, started with manufacturing, R&D and sales of X-ray machines, clinical lab device, hematology analyzer, ventilator and reagents.
In the field of lab equipment, we have owned good reputation in both domestic and overseas market. Our portfolio includes Elisa reader, Elisa washer, biochemistry analyzer, hematology analyzer, electrolyte analyzer and its applicable reagents. Recently our R&D department developed new product which is very advanced and with nice appearance, electrolyte blood gas analyzer. We have achieved good market share and got nice feedback till now, especially Elisa reader which has CE certificate.
Enzyme-linked immunosorbent assay (ELISA), is a popular format of a "wet-lab" type analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.
Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[8] or West Nile virus). It has also found applications in the food industry in detecting potential food allergens, such as milk, peanuts, walnuts, almonds, and eggs.[9] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs.
Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[8] or West Nile virus). It has also found applications in the food industry in detecting potential food allergens, such as milk, peanuts, walnuts, almonds, and eggs.[9] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs.
The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400 times and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme.
ELISA readers or micro plate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein. A spectrophotometer measures ultraviolet and visible light.
Additionally, ELISA plate readers can also measure fluorescence and luminescence. Chemical dyes fluoresce or emit one color or wavelength when exposed to light. The amount of reflection, absorption and the color identify, and measure the amount of a substance.
ELISA readers were designed for measuring antibody tests. They worked so well, the machine has been adapted to other purposes. Researchers use them for protein and enzyme assays. They are also used for HIV detection and quantitation of nucleic acids.
To use a spectrophotometer or ELISA plate reader, the molecule must be dissolved in solution. A spectrophotometer requires between 400 micro-liters and four milliliters, depending on the manufacturer and model. An ELISA plate reader needs about two to 100 micro-liters; ELISA plate readers use much less of a sample to get a result.
ELISA plate readers measure more samples in a shorter period of time. A spectrophotometer measures one to six samples at a time. Typically, an ELISA plate measures 96 wells in an equivalent amount of time.
ELISA readers or micro plate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein. A spectrophotometer measures ultraviolet and visible light.
Additionally, ELISA plate readers can also measure fluorescence and luminescence. Chemical dyes fluoresce or emit one color or wavelength when exposed to light. The amount of reflection, absorption and the color identify, and measure the amount of a substance.
ELISA readers were designed for measuring antibody tests. They worked so well, the machine has been adapted to other purposes. Researchers use them for protein and enzyme assays. They are also used for HIV detection and quantitation of nucleic acids.
To use a spectrophotometer or ELISA plate reader, the molecule must be dissolved in solution. A spectrophotometer requires between 400 micro-liters and four milliliters, depending on the manufacturer and model. An ELISA plate reader needs about two to 100 micro-liters; ELISA plate readers use much less of a sample to get a result.
ELISA plate readers measure more samples in a shorter period of time. A spectrophotometer measures one to six samples at a time. Typically, an ELISA plate measures 96 wells in an equivalent amount of time.
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